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A senescence β-galactosidase assay was conducted using the senescence β-galactosidase staining kit (Cell Signaling Technology, 9860) according to the manufacturer’s instructions, and then stained cells were imaged using a ZEISS Primovert inverted microscope (Carl Zeiss Microscopy)

electrolyte balance

Milligram:5mg

Timing within the day: Every protocol and available study used evening administration