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In brief, 10 μl of cytosolic lysate was added to wells in the 96 well plate and then 210 μl of mixture containing 190 μl of assay buffer (50 mM Tris-HCl, pH 7.0 and 600 mM KCl), 10 μl of enzyme dilution buffer (20 mM Tris-HCl, pH 7.0 and 500 mM sucrose), and 10 μl of 0.22 mM ferrocytochrome c substrate solution was added to the wells

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