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Briefly, 50 μL of caspase assay mixture containing 150 mM HEPES, pH 7.4, 450 mM NaCl, 150 mM KCl, 30 mM MgCl 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 μM caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells