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[13] The first reaction of the carnitine shuttle is a two-step process catalyzed by a family of isozymes of acyl-CoA synthetase that are found in the outer mitochondrial membrane, where they promote the activation of fatty acids by forming a thioester bond between the fatty acid carboxyl group and the thiol group of coenzyme A to yield a fatty acyl–CoA

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Carnitine (ALC) is a nutrient that occurs naturally in the body and is fundamentally important for energy generation by the mitochondria energy factories in our cells