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In particular with the CRISPRa approach, the transcriptional activators that are fused to the dCas9 enzyme will increase the length of the construct, such that a combination of sgRNA and dCas9-transcriptional activators will be too large to load onto a single AAV vector

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Complete rest from aggravating activities for at least the first 2 to 4 weeks of treatment allows the enhanced healing response to gain traction before gradually reintroducing load