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To compare the expression of these genes in response to H 2 O 2 , we measured by qPCR the transcriptional level of each one in the pneumococcal R801strain (derivate of the R6 strain) [31] exposed at 20 mM H 2 O 2 for 30 min, and referred to gyrB as internal control [26]

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By comparing the migration patterns of bound and free DNA, researchers can determine the binding affinity and specificity of the protein for the DNA sequence

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