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Excessive consumption of alcohol is associated with health conditions, such as liver disease, cardiovascular disease, certain types of cancer, diabetes, anxiety, and depression

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The next day, 10% FBS containing RPMI 1640 medium was added, and cells were treated with 10 ng/ml TNF-ɑ, with and without NR 250 μM, and incubated for 24 h

especially in the liver.