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Human protocols circulating in self-experimentation communities use 1 to 2 mg/kg subcutaneously, but these doses have no clinical trial support and the human safety profile is entirely unknown

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Common problems in GST tag protein purification Non-binding or weak binding of GST tag proteins Possible reasons for inefficient elution of GST tag proteins High level of heteroproteins in purified product Conclusion Generally speaking, purity of GST tag proteins via one-step affinity chromatography will be higher than that of His tag protein via one-step purification